GeoMx Digital Spatial Profiler (DSP) Protocol v2 - University of Minnesota TMCs
Laura J Niedernhofer, David A Bernlohr
Published: 2024-03-27 DOI: 10.17504/protocols.io.8epv5r7k6g1b/v2
AI 解读 浏览 1328
Abstract
The nanoString GeoMx® Digital Spatial Profiler (DSP) is a platform that allows high-plex profiling at the protein and RNA level, providing spatial and temporal assessment of tumors in frozen or formalin-fixed paraffin-embedded limited tissue sample.
The following protocols/manuals were used at the University of Minnesota TMCs in collaboration with the University of Minnesota Genomics Center.
Steps
Slide Preparation
1.
GeoMx DSP Manual Slide Preparation User Manual
MAN-10150-04.pdf
ROI Acquisition
2.
GeoMx DSP Instrument User Manual
MAN-10152-04.pdf
Library Preparation
3. Sequence with the read format 27,8,8,27
GeoMx DSP NGS Readout User Manual
MAN-10153-04.pdf
Note
FASTQ Generation
4.
BCL data from Illumina sequencer is demultiplexed and converted into FASTQ format using bcl2fastq version 2.20.0
推荐阅读
Nature Protocols
Protocols IO
Current Protocols
