Click chemistry: a transformative technology in nuclear medicine

Samantha M. Sarrett, Brian M. Zeglis, David Bauer, Jason S. Lewis

Published: 2023-04-25 DOI: 10.1038/s41596-023-00825-8

click chemistry

nuclear medicine

radiopharmaceutical chemistry

azide-alkyne cycloaddition

copper-catalyzed reaction

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Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

Bioengineering human intestinal mucosal grafts using patient-derived organoids, fibroblasts and scaffolds

Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

Neuroscience robotics for controlled induction and real-time assessment of hallucinations

Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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The 2022 Nobel Prize in Chemistry was awarded to Professors K. Barry Sharpless, Morten Meldal and Carolyn Bertozzi for their pioneering roles in the advent of click chemistry. Sharpless and Meldal worked to develop the canonical click reaction—the copper-
The CuAAC reaction
The SPAAC reaction
‘Next-generation’ click reactions
- Outlook
References
ACKNOWLEDGMENTS

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Click chemistry: a transformative technology in nuclear medicine

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